A CRISPR blog / bibliography

New developments in CRISPR technology, with a focus on mouse and human cell applications.

Showing posts with label germline. Show all posts
Showing posts with label germline. Show all posts

Monday, September 21, 2015

Link to a timeline of recent discourse on #CRISPR human germline gene editing.

I've started a separate blog about human germline gene editing.  Hope you like it.  My first post is a list of links to recent news items on the topic.
Posted by Douglas Mortlock at 9:37 AM No comments:
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Labels: ethics, germline
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  • Links to a few handy CRISPR target tools.

Please check out my blog about human germline gene editing.

Doug Mortlock

Doug Mortlock

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adapters albino algorithm assay award binding Cas9 cleavage core Cpf1 Cre decomposition disease donor E1B55K E4orf6 editing efficiency electroporation embryo ethics gene therapy genotyping germline GFP guidelines HDR heteroduplex homology arm human cells in vivo indel inducible insertion iPS knockdown mutation NHEJ off-target optimization PAGE PAM photoactivatable piggyBAC plasmid pronuclear injection protocol protospacer protospacer-adjacent motif PX330 rat reporter RGEN-RFLP RNA RNA vs DNA SCR7 seed sequencing sgRNA ssODN stem cells surveyor assay T7 assay target transgenic tyrosinase whole-genome sequencing zygote

About the author

Doug Mortlock is a geneticist, molecular biologist and development biologist at Vanderbilt University.
@Doug_Mortlock

The Cas9 CRISPR target

The Cas9 CRISPR target

CRISPR Links

  • Vanderbilt TMESCSR (Transgenic mouse core)
  • Zhang lab (MIT) CRISPR Resources page
  • Zhang lab (MIT) CRISPR target & off-target search
  • ZiFiT CRISPR target & off-target search
  • COSMID off-target search
  • TIDE chromatogram analysis tool
  • SSC - Sequence Scan for CRISPR

Sweet.

Sweet.

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