A Mouse Geneticist's Practical Guide to CRISPR Applications. Singh P, Schimenti JC, Bolcun-Filas E. Genetics. 2014 Sep 29. pii: genetics.114.169771. [Epub ahead of print]
Monday, October 13, 2014
Outstanding #CRISPR review: "A mouse geneticist's practical guide to CRISPR applications".
Far above Cayuga's waters - my alma mater! - there are some excellent scientists using CRISPR to engineer mutations in mice. This paper is from John Schimenti's lab at Cornell and I believe it contains the most thorough review of mouse CRISPR engineering to date.
A Mouse Geneticist's Practical Guide to CRISPR Applications. Singh P, Schimenti JC, Bolcun-Filas E. Genetics. 2014 Sep 29. pii: genetics.114.169771. [Epub ahead of print]
More than merely a review article, it has some additional new data from this group. They tested whether inhibition of the NHEJ pathway could enhance efficiency of CRISPR-mediated homology-directed repair, since these pathways compete following CRISPR cleavage; this was based on similar experiments done in Drosophila using ZFNs to do HDR. The answer seems to be yes, it helps in mouse embryos as well. The compound they used was SCR7, an inhibitor of ligase IV (a key player in NHEJ). Crucially, it seems that SCR7 can be applied directly to mouse embryo culture media with minimal toxicity. Go to Table 2 for the result suggesting a shift in balance from predominantly NHEJ alleles toward more HDR alleles.
A Mouse Geneticist's Practical Guide to CRISPR Applications. Singh P, Schimenti JC, Bolcun-Filas E. Genetics. 2014 Sep 29. pii: genetics.114.169771. [Epub ahead of print]
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