Tuesday, April 1, 2014

CRISPR/Cas9-mediated knock-ins in zebrafish

This paper demonstrates targeted insertion by non-homologous-end-joining - and thus, not by homologous recombination - in fish embryos, and with germline transmission.  It's still unclear to me how efficient this will be to generate germline transmission in mice, but this is a promising step.

 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31.
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.
Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F.

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